当社開発の細菌・アーキア一括プライマーセット

当社開発のアンプリコンシーケンス解析用プライマーセット(細菌・アーキア一括)1)は、2017年に腸内微生物叢解析のGoldstandard Method(s) として紹介されました2,3)。今日まで、腸内環境はもちろん自然環境分野の論文でも多く採用されています4-15)。

当社では、細菌のみを対象としたアンプリコンシーケンス解析も承っておりますが、腸内のメタン産生やバイオリアクターのアンモニア酸化など、その環境の微生物群集がもつ生理的機能や多様性をより精確に捉えるという観点から、細菌・アーキア一括プライマーセットでの解析をお奨めしております。

  • (1)

    Takahashi S, Tomita J, Nishioka K, Hisada T, Nishijima M. Development of a prokaryotic universal primer for simultaneous analysis of Bacteria and Archaea using next-generation sequencing. PLoS One 2014;9:e105592.
  • (2)

    Rojo D, Mendez-Garcia C, Raczkowska BA, Bargiela R, Moya A et al. Exploring the human microbiome from multiple perspectives: factors altering its composition and function. FEMS Microbiol Rev 2017;41:453-478.
  • (3)

    Ferrer M, Raczkowska BA, Martinez-Martinez M, Barbas C, Rojo D. Phenotyping of gut microbiota: Focus on capillary electrophoresis. Electrophoresis 2017;38:2275-2286.
  • (4)

    Hari AR, Katuri KP, Logan BE, Saikaly PE. Set anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cells. Sci Rep 2016;6: 38690.
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    Barthels C, Ogrinc A, Steyer V, Meier S, Simon F et al. CD40-signalling abrogates induction of RORγt+ Treg cells by intestinal CD103+ DCs and causes fatal colitis. Nat Commun 2017;8: 14715.
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    McAnulty MJ, Poosarla VG, Kim K-Y, Jasso-Chavez R, Logan B et al. Electricity from methane by reversing methanogenesis. Nat Commun 2017;8:15419.
  • (7)

    Cahill AG, Steelman CM, FordeO, Kuloyo O, Ruff SE et al. Mobility and persistence of methane in groundwater in a controlled-release field experiment. Nat Geosci 2017;10:289–294.
  • (8)

    Li M, Zhou H, Pan X, Xu T, Zhang Z et al. Cassava foliage affects the microbial diversity of Chinese indigenous geese caecum using 16S rRNA sequencing. Sci Rep 2017;7: 45697.
  • (9)

    Kunath BJ, Delogu F, Naas AE, Arntzen MØ, Eijsink VG et al. From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J 2019;13:603-617.
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    Müller H, Marozava S, Probst AJ, Meckenstock RU. Groundwater cable bacteria conserve energy by sulfur disproportionation. ISME J 2020;14:623-634.
  • (11)

    Morella NM, Weng F C-H, Joubert PM, Metcalf CJE, Lindow S et al. Successive passaging of a plant-associated microbiome reveals robust habitat and host genotype-dependent selection. Proc Natl Acad Sci 2020;117:148-1159.
  • (12)

    Fernández-González AJ, Cardoni M, Cabanás C G-L, Valverde-Corredor A, Villadas PJ et al. Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive. Microbiome 2020;8:11.
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    Cichocki N, Hübschmann T, Schattenberg F, Kerckhof FM, Overmann J et al. Bacterial mock communities as standards for reproducible cytometric microbiome analysis. Nat Protoc 2020;15:2788-2812.
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    Konuma T, Kohara C, Watanabe E, Takahashi S, Ozawa G, Suzuki K et al. Reconstitution of Circulating Mucosal-Associated Invariant T Cells after Allogeneic Hematopoietic Cell Transplantation: Its Association with the Riboflavin Synthetic Pathway of Gut Microbiota in Cord Blood Transplant Recipients. J Immunol 2020;204:1462-1473.
  • (15)

    Holman LE, De Bruyn M, Creer S, Carvalho G, Robidart J et al. Animals, protists and bacteria share marine biogeographic patterns. Nat Ecol Evol 2021; https://doi.org/10.1038/s41559-021-01439-7